Laboratory results will be reported as reactive or nonreactive. If the donation is reactive, the sample will be tested for viral markers to identify the virus that infected the donor. If the donation is nonreactive, the sample will be tested for antibody to the virus. Confirmatory tests should be performed as soon as possible, preferably within 24 hours. If confirmatory testing is positive and the donor is a new donor for that test, further tests will be performed. The testing performed is based on the screening test result and the volume of sample available. If the sample is greater than the minimum volume, the confirmatory test will be performed on the entire sample.
Note: Some tests have minimum volume requirements; if the minimum volume cannot be met, the result will be considered negative. If the sample is insufficient, the result will be considered nonreactive. If the screening and confirmatory results are discordant, further testing will be performed.
Screening (Viral: HBV DNA, HCV RNA, anti-HCV)
A BSL2 or BSL3 facility is requested; labs that are not BSL2 or BSL3 will need to be approved. The lab will determine the appropriate test protocol for testing. A minimum volume of the sample may be required for shipping.
Test collection and shipping instructions:
You will be asked to provide a blood sample (the volume depends on the lab) and you will be asked to provide consent to have a sample sent to the lab. You can mail a sample, or you may be required to come to the lab.
HCV RNA and anti-HCV are the first viral markers to circulate in an individual infected with HCV. Seroconversion to HCV (anti-HCV) occurs 1-4 weeks after infection (5% or less). HCV antibody (anti-HCV) is detectable in the blood at the onset of symptoms for those adults who develop symptoms. HCV-RNA testing can detect HCV-RNA in serum before the appearance of anti-HCV.
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